Guy J R, Bisby M A
Exp Neurol. 1983 Dec;82(3):706-10. doi: 10.1016/0014-4886(83)90092-4.
The difficulties involved in measuring axonal transport velocity for phospholipid in mammalian axons have been overcome by using a reversible local cold-block technique which causes a local accumulation of labeled phospholipid in rat sciatic nerve that follows injection of [methyl 3H]choline chloride into the L5 dorsal root ganglion. On rewarming the nerve a distinct front of [3H]-activity moves distally from the cold-block site at 17.8 mm X h-1, a velocity not significantly different from that previously determined for protein. This observation provides further support for the hypothesis that fast axonal transport conveys membranes assembled in the cell body.
通过使用可逆性局部冷阻断技术，克服了测量哺乳动物轴突中磷脂轴突运输速度所涉及的困难。该技术会使注入L5背根神经节的[甲基³H]氯化胆碱后，大鼠坐骨神经中标记磷脂出现局部积累。当神经复温时，一个明显的[³H]活性前沿以17.8毫米×小时⁻¹的速度从冷阻断部位向远端移动，该速度与先前测定的蛋白质运输速度无显著差异。这一观察结果为快速轴突运输传递在细胞体中组装的膜这一假说提供了进一步支持。
