Suzuki M R, Kikuyama S
Gen Comp Endocrinol. 1983 Nov;52(2):272-8. doi: 10.1016/0016-6480(83)90122-3.
The effect of aldosterone and corticosterone on T3 binding to the nuclear fraction of the tail fin of bullfrog tadpoles was investigated in vitro. Both corticoids (10(-7) - 10(-5) M) added to the incubation medium increased T3 binding in a dose-dependent manner. The aldosterone-induced increase in T3-binding was blocked by cycloheximide, an inhibitor of protein synthesis, and actinomycin D, an inhibitor of RNA synthesis. Scatchard analysis revealed that 10(-6) M aldosterone and corticosterone increased the maximum binding capacity for T3 by 60 and 41%, respectively, and that the corticoids did not alter the value of the dissociation constant. The significance of the finding is discussed in relation to metamorphosis.
体外研究了醛固酮和皮质酮对牛蛙蝌蚪尾鳍细胞核部分结合T3的影响。添加到孵育培养基中的两种皮质激素（10^(-7) - 10^(-5) M）均以剂量依赖性方式增加T3结合。醛固酮诱导的T3结合增加被蛋白质合成抑制剂环己酰亚胺和RNA合成抑制剂放线菌素D阻断。Scatchard分析表明，10^(-6) M的醛固酮和皮质酮分别使T3的最大结合能力提高了60%和41%，且皮质激素未改变解离常数的值。结合变态发育讨论了该发现的意义。
