Incomplete expression of the MOPC 460 idiotype in the sera of BALB/c mice immunized either with DNP antigen or with anti-idiotypic antibodies.

The MOPC 460 idiotype, as defined by polyclonal probes, has been described as a recurrent marker among the anti-DNP antibodies synthetized by IghCa mice. In this paper, we demonstrate, using syngeneic monoclonal anti-idiotypic probes, that only a part of the idiotopes of this idiotype are indeed recurrently expressed in BALB/c mice (IghCa) after immunization with DNP antigen. We will also show that the immunization of BALB/c mice with monoclonal anti-idiotypic antibody specific for the recurrent determinant results firstly in the synthesis of anti-DNP antibodies and secondly in the expression of the same recurrent M460 idiotope present on a part of induced anti-DNP molecules. Contrary to this, the immunization with the monoclonal anti-idiotypic antibody specific for the private idiotope never resulted in the synthesis of anti-DNP antibodies. These results clearly suggest that, after DNP or anti-idiotypic immunization, the M460 idiotype is not expressed in its entirety.