Sanchez P, Le Guern C, Phalente L, Barbier E, Buttin G, Cazenave P A
Mol Immunol. 1982 Jul;19(7):885-92. doi: 10.1016/0161-5890(82)90354-6.
Eight syngeneic anti-idiotypic hybridomas (IDMs) have been obtained against the BALB/c myeloma protein MOPC460 which displays anti-TNP activity. The study of their anti-idiotype specificity allowed us to distinguish them into two groups which define the presence of at least two idiotypic determinants or idiotopes in the MOPC460 idiotype. The biochemical analysis of the monoclonal antibodies is consistent with this dichotomy. This analysis, in fact, showed a striking correlation between anti-idiotypic specificity and biochemical characteristics of the monoclonal antibodies. Consequently, the idiotypic specificities of three of these hybridomas were studied. In accordance with what is expected, our results clearly indicate a strong idiotypic similarity for hybridomas belonging to the same group and a lack of idiotypic cross-reactivity.
已获得八种针对显示抗三硝基苯（TNP）活性的BALB/c骨髓瘤蛋白MOPC460的同基因抗独特型杂交瘤（IDM）。对其抗独特型特异性的研究使我们能够将它们分为两组，这表明在MOPC460独特型中至少存在两个独特型决定簇或独特位。单克隆抗体的生化分析与这种二分法一致。事实上，该分析表明抗独特型特异性与单克隆抗体的生化特性之间存在显著相关性。因此，研究了其中三种杂交瘤的独特型特异性。正如预期的那样，我们的结果清楚地表明，属于同一组的杂交瘤具有很强的独特型相似性，并且不存在独特型交叉反应性。
