胰岛素与脂质体外表面的共价连接。
Covalent attachment of insulin to the outer surface of liposomes.
作者信息
Cantenys D, Creach Y, Pradayrol L, Hollande E, Massol M
出版信息
Biochem Biophys Res Commun. 1983 Dec 16;117(2):399-405. doi: 10.1016/0006-291x(83)91214-7.
We describe a method for covalent binding of insulin to the outer surface of multilamellar liposomes loaded with spin label. Encapsulation of the label Tempocholine-nitroxide within the aqueous phases of liposomes is controlled by Electron Spin Resonance. The binding of insulin is performed using the Carlsson's heterobifunctional reagent: N-succinimidyl 3-(2-pyridyldithio) propionate. The coupling method results in efficient attachment of 2. 64.10(-4) mole of insulin per mole of phospholipid; the integrity of these vesicles is not modified as confirmed by spin resonance analysis. Moreover, the liposome-coupled insulin retains its antigenic specificity as shown by radioimmunoassays.
我们描述了一种将胰岛素共价结合到负载自旋标记的多层脂质体外表面的方法。通过电子自旋共振控制自旋标记Tempo胆碱 - 氮氧化物在脂质体水相中的包封。使用卡尔森异双功能试剂：N - 琥珀酰亚胺基3 - （2 - 吡啶二硫基）丙酸酯进行胰岛素的结合。该偶联方法每摩尔磷脂可有效连接2.64×10⁻⁴摩尔胰岛素；自旋共振分析证实这些囊泡的完整性未被改变。此外，如放射免疫测定所示，脂质体偶联的胰岛素保留了其抗原特异性。
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