Banks-Schlegel S P, Harris C C
Cancer Res. 1984 Mar;44(3):1153-7.
When compared to normal esophageal epithelium, marked alterations in keratin protein and cross-linked envelope expression were found in human esophageal carcinomas. Examination of the pattern of keratin proteins extracted from either several primary esophageal tumors or carcinomas xenotransplanted in nude mice revealed a dramatic reduction in the amount of keratin protein, especially in the Mr 52,000 to 61,000 range. In seven of eight of the primary tumors, the major Mr 52,000 and Mr 61,000 esophageal keratins were not detected, and the remaining tumor exhibited a marked reduction in these two keratins. The major Mr 57,000 and minor Mr 59,000 esophageal keratins were found in varying but reduced amounts in the different tumors. The major Mr 57,000 keratin seemed to be the most conserved keratin of this intermediate-molecular-weight keratin class (Mr 52,000 to 61,000). In contrast, the lower-molecular-weight keratins (Mr 46,000 to 50,500) were usually conserved in the carcinoma cells and were present at levels approximating that of the nontransformed counterpart. The minor Mr 37,000 and 44,000 keratins from normal esophageal epithelium were retained in the tumor cells but often in reduced amounts. The expression of another differentiated function, cross-linked envelopes, in the carcinoma cells varied from unimpaired to severely restricted capacity to form envelopes. In conclusion, specific alterations in keratin protein and cross-linked envelope expression were found in human esophageal carcinomas.
与正常食管上皮相比，在人类食管癌中发现角蛋白和交联包膜表达有明显改变。对从几种原发性食管肿瘤或移植于裸鼠的癌组织中提取的角蛋白模式进行检测，结果显示角蛋白的量显著减少，尤其是在分子量52,000至61,000范围内。在8个原发性肿瘤中的7个中，未检测到主要的分子量52,000和61,000的食管角蛋白，而其余肿瘤中这两种角蛋白明显减少。不同肿瘤中分子量57,000的主要食管角蛋白和分子量59,000的次要食管角蛋白含量不同但均减少。分子量57,000的主要角蛋白似乎是该中等分子量角蛋白类别（分子量52,000至61,000）中最保守的角蛋白。相比之下，低分子量角蛋白（分子量46,000至50,500）通常在癌细胞中得以保留，其含量与未转化的对应细胞相近。正常食管上皮中的次要分子量37,000和44,000的角蛋白在肿瘤细胞中也有保留，但含量常常减少。癌细胞中另一种分化功能交联包膜的表达，从形成包膜的能力未受影响到严重受限不等。总之，在人类食管癌中发现了角蛋白和交联包膜表达的特异性改变。
