Burbach J P, Wiegant V M
FEBS Lett. 1984 Jan 30;166(2):267-72. doi: 10.1016/0014-5793(84)80093-9.
alpha-Endorphin and gamma-endorphin, two closely related peptides of the pro-opiomelanocortin family with characteristic biological activities, were purified to homogeneity from single human pituitary glands and chemically identified. Isolation of the peptides was based on size fractionation by Sephadex G-75 chromatography followed by two HPLC steps using reverse-phase and paired-ion reverse-phase systems and was monitored by radioimmunoassay. During the isolation procedure alpha- and gamma-endorphin-sized material behaved chromatographically and immunologically indistinguishably from synthetic alpha- and gamma-endorphin. The amino acid composition and NH2-terminus of isolated peptides demonstrated their identity as authentic alpha-endorphin and gamma-endorphin. Acetylated forms were absent. In addition, evidence is provided that large forms with alpha- and gamma-endorphin immunoreactivity detected during gel filtration are human lipotropin-(1-74) and -(1-75), respectively. The data substantiate that alpha-endorphin and gamma-endorphin exist as endogenous peptides in the human pituitary gland.
α-内啡肽和γ-内啡肽是促阿片黑素细胞皮质素家族中两个密切相关的具有特征性生物活性的肽，它们从单个人类垂体中纯化至同质，并进行了化学鉴定。肽的分离基于通过Sephadex G-75色谱进行的尺寸分级，随后使用反相和离子对反相系统进行两步高效液相色谱，并通过放射免疫测定法进行监测。在分离过程中，α-和γ-内啡肽大小的物质在色谱行为和免疫反应上与合成的α-和γ-内啡肽没有区别。分离出的肽的氨基酸组成和NH2末端证明它们分别是真正的α-内啡肽和γ-内啡肽。不存在乙酰化形式。此外，有证据表明在凝胶过滤过程中检测到的具有α-和γ-内啡肽免疫反应性的大形式分别是人促脂素-(1-74)和-(1-75)。数据证实α-内啡肽和γ-内啡肽作为内源性肽存在于人类垂体中。
