Shott R J, Lee J J, Britten R J, Davidson E H
Dev Biol. 1984 Feb;101(2):295-306. doi: 10.1016/0012-1606(84)90143-x.
Molecular probes that individually recognize the 3' nontranslated regions of six actin genes were utilized in RNA gel blot hybridizations to detect RNAs complementary to each gene in embryonic and adult tissues of Strongylocentrotus purpuratus. In addition the probes were used in DNA excess filter hybridizations to estimate the relative contribution of the different actin genes. All six genes produce relatively stable mRNAs, and each displays a characteristic and distinct pattern of expression. On the basis of their expression in the egg, early embryos, or in adult coelomocytes, it is concluded that genes termed CyI, CyIIa, CyIIb, CyIIIa, and CyIIIb encode cytoskeletal actin proteins. Actin gene M gives rise to mRNAs that are found only in tissues containing muscle. Actin genes CyI, CyIIa, CyIIb, and M are expressed in both adult and embryonic tissues, giving rise to transcripts 2.1-2.2 kb in length. Expression of genes CyIIIa and CyIIIb is confined to the embryo. Gene CyIIIa provides the major embryonic actin mRNA, which is 1.8 kb in length. Three of the cytoskeletal actin genes are linked over a 30-kb distance in the S. purpuratus genome. We show that the actin genes included in this linkage group are not coordinately expressed.
利用能分别识别六种肌动蛋白基因3'非翻译区的分子探针，进行RNA凝胶印迹杂交，以检测与紫海胆胚胎和成年组织中每个基因互补的RNA。此外，这些探针还用于DNA过量滤膜杂交，以估计不同肌动蛋白基因的相对贡献。所有六个基因都产生相对稳定的mRNA，并且每个基因都显示出独特且不同的表达模式。根据它们在卵、早期胚胎或成年体腔细胞中的表达情况，得出结论：称为CyI、CyIIa、CyIIb、CyIIIa和CyIIIb的基因编码细胞骨架肌动蛋白。肌动蛋白基因M产生的mRNA仅在含有肌肉的组织中发现。肌动蛋白基因CyI、CyIIa、CyIIb和M在成年和胚胎组织中均有表达，产生长度为2.1 - 2.2 kb的转录本。基因CyIIIa和CyIIIb的表达仅限于胚胎。基因CyIIIa提供主要的胚胎肌动蛋白mRNA，其长度为1.8 kb。在紫海胆基因组中，三个细胞骨架肌动蛋白基因在30 kb的距离内连锁。我们表明，包含在该连锁群中的肌动蛋白基因并非协同表达。
