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猪蛔虫肠周液中某些酶的活性

Activities of some enzymes in the perienteric fluid of Ascaris suum.

作者信息

Zenka J, Prokopic J

出版信息

Folia Parasitol (Praha). 1983;30(4):373-6.

PMID:6198263
Abstract

Activities of 12 enzymes (amylase, lipase, cholinesterase, nonspecific carboxyl esterase, lactate dehydrogenase (LDH), alkaline phosphatase, glutamate-oxalacetate transaminase (GOT), glutamate-pyruvate transaminase (GPT), gamma-glutamyl transferase (gamma-GT), leucine aminopeptidase (LAP), malate dehydrogenase (MDH) and peroxidase) were determined in the perienteric fluid and homogenate of Ascaris suum. With the exception of amylase, all activities were higher in the homogenate than in the perienteric fluid. The enzyme activities in the perienteric fluid were then compared with those in the human serum. Comparable activities were demonstrated for LDH, LAP, lipase and alkaline phosphatase, markedly higher activities in perienteric fluid were demonstrated for MDH, GOT, GPT and amylase, and much lower for cholinesterase. No gamma-GT activity was detected in the perienteric fluid.

摘要

测定了猪蛔虫肠周液和匀浆中12种酶(淀粉酶、脂肪酶、胆碱酯酶、非特异性羧酸酯酶、乳酸脱氢酶(LDH)、碱性磷酸酶、谷草转氨酶(GOT)、谷丙转氨酶(GPT)、γ-谷氨酰转移酶(γ-GT)、亮氨酸氨肽酶(LAP)、苹果酸脱氢酶(MDH)和过氧化物酶)的活性。除淀粉酶外,匀浆中的所有酶活性均高于肠周液。然后将肠周液中的酶活性与人血清中的酶活性进行比较。LDH、LAP、脂肪酶和碱性磷酸酶表现出可比的活性,MDH、GOT、GPT和淀粉酶在肠周液中的活性明显更高,胆碱酯酶的活性则低得多。在肠周液中未检测到γ-GT活性。

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