Law S W, Brewer H B
Proc Natl Acad Sci U S A. 1984 Jan;81(1):66-70. doi: 10.1073/pnas.81.1.66.
The cDNA clones encoding the precursor form of human liver apolipoprotein A-I (apoA-I), preproapoA-I, have been isolated from a cDNA library. A 17-base synthetic oligonucleotide based on residues 108-113 of apoA-I and a 26-base primer-extended, dideoxynucleotide-terminated cDNA were used as hybridization probes to select for recombinant plasmids bearing the apoA-I sequence. The complete nucleic acid sequence of human liver preproapoA-I has been determined by analysis of the cloned cDNA. The sequence is composed of 801 nucleotides encoding 267 amino acid residues. PreproapoA-I contains an 18-amino-acid prepeptide and a 6-amino-acid propeptide connected to the amino terminus of the 243-amino acid mature apoA-I. Southern blotting analysis of chromosomal DNA obtained from peripheral blood indicated the apoA-I gene is contained in a 2.1-kilobase-pair Pst I fragment and there is no gross difference in structural organization between the normal apoA-I gene and the Tangier disease apoA-I gene.
编码人肝脏载脂蛋白A-I（apoA-I）前体形式即前原载脂蛋白A-I的cDNA克隆已从一个cDNA文库中分离出来。基于apoA-I第108 - 113位残基的17个碱基的合成寡核苷酸以及一个26个碱基的引物延伸、双脱氧核苷酸终止的cDNA被用作杂交探针，以筛选带有apoA-I序列的重组质粒。通过对克隆的cDNA进行分析，已确定人肝脏前原载脂蛋白A-I的完整核酸序列。该序列由801个核苷酸组成，编码267个氨基酸残基。前原载脂蛋白A-I包含一个18个氨基酸的前肽和一个与243个氨基酸的成熟apoA-I氨基末端相连的6个氨基酸的肽原。对外周血获得的染色体DNA进行的Southern印迹分析表明，apoA-I基因包含在一个2.1千碱基对的Pst I片段中，正常apoA-I基因与丹吉尔病apoA-I基因在结构组织上没有明显差异。
