鸟嘌呤衍生物对雌激素预处理大鼠脊柱前凸行为的影响。

Effect of guanine derivatives on lordosis behavior in estrogen primed rats.

作者信息

Fernández-Guasti A, Rodríguez-Manzo G, Beyer C

出版信息

Physiol Behav. 1983 Nov;31(5):589-92.

PMID:6198669

Abstract

The effect of systemic administration of guanosine, cGMP, dipalmitoyl (dp) cGMP, 5'GMP and 5'GDP on lordosis behavior was studied in ovariectomized, estradiol benzoate (EB) primed rats (4 micrograms/rat). EB per se induced only weak lordosis behavior. Free cGMP (2.0 mg/rat); dp cGMP (1.0, 2.0 and 4.0 mg/rat) and 5'GMP (2.0 and 4.0 mg/rat) induced significant lordosis behavior in ovariectomized, estrogen-primed rats. Dp cGMP and 5'GMP (2.0 mg/rat), also induced lordosis behavior in ovariectomized, adrenalectomized estrogen primed rats. Lordosis behavior elicited with 2.0 mg/rat dp cGMP was blocked with 8.0 mg/rat methyl-isobutylxanthine (MIX), a phosphodiesterase inhibitor, suggesting that dp cGMP stimulated lordosis through its conversion to 5'GMP. Results are interpreted in terms of the activation of adenylate cyclase-cAMP systems by guanine nucleotides.

摘要

在切除卵巢并用苯甲酸雌二醇（EB，4微克/只大鼠）预处理的大鼠中，研究了全身给予鸟苷、环磷酸鸟苷（cGMP）、二棕榈酰（dp）cGMP、5'-鸟苷酸（5'GMP）和5'-鸟苷二磷酸（5'GDP）对脊柱前凸行为的影响。单独使用EB仅诱导出较弱的脊柱前凸行为。游离cGMP（2.0毫克/只大鼠）、dp cGMP（1.0、2.0和4.0毫克/只大鼠）以及5'GMP（2.0和4.0毫克/只大鼠）在切除卵巢且经雌激素预处理的大鼠中诱导出显著的脊柱前凸行为。dp cGMP和5'GMP（2.0毫克/只大鼠）在切除卵巢且肾上腺切除并经雌激素预处理的大鼠中也诱导出脊柱前凸行为。用磷酸二酯酶抑制剂8.0毫克/只大鼠甲基异丁基黄嘌呤（MIX）可阻断2.0毫克/只大鼠dp cGMP引发的脊柱前凸行为，这表明dp cGMP通过转化为5'GMP来刺激脊柱前凸。研究结果根据鸟嘌呤核苷酸对腺苷酸环化酶-cAMP系统的激活作用来解释。