Barnhill R L, Parkinson E K, Ryan T J
Br J Dermatol. 1984 Mar;110(3):273-81. doi: 10.1111/j.1365-2133.1984.tb04632.x.
Conditioned media from three different strains of human epidermal keratinocytes in culture were assayed on the chorioallantoic membrane of the chick embryo. Vascular responses were examined 4 days later stereo-microscopically and compared with controls--unconditioned medium and medium incubated with 3T3 cells. Specimens were also collected after 1, 2, 3 and 4 days for serial histological examination. Media from all three strains of keratinocytes stimulated statistically significant vascular growth relative to controls. Leukocytic infiltration was not demonstrated histologically at any time. In addition, quantitation of ectodermal epithelial hyperplasia in experimental groups did not reveal any significant difference when compared with controls. These results have demonstrated that epidermis may directly encourage vascular growth in the absence of significant inflammation.
对培养的三种不同人类表皮角质形成细胞系的条件培养基在鸡胚尿囊绒膜上进行检测。4天后，用体视显微镜检查血管反应，并与对照组——未处理的培养基和与3T3细胞一起孵育的培养基进行比较。在第1、2、3和4天后也收集标本进行连续组织学检查。相对于对照组，来自所有三种角质形成细胞系的培养基均刺激了具有统计学意义的血管生长。在任何时候组织学检查均未发现白细胞浸润。此外，与对照组相比，实验组中外胚层上皮增生的定量分析未显示出任何显著差异。这些结果表明，在无明显炎症的情况下，表皮可能直接促进血管生长。
