Byers S W, Qasba P K, Paulson H L, Dym M
Biol Reprod. 1984 Feb;30(1):171-8. doi: 10.1095/biolreprod30.1.171.
The immunocytochemical localization of the milk protein alpha-lactalbumin in the male reproductive tract is described. Using a primary antiserum raised against highly purified rat milk alpha-lactalbumin, specific staining was consistently shown in the supranuclear Golgi region of the principal cells of the proximal caput epididymidis but only occasionally in epithelial cells from other regions of the duct. Staining was also found in the epididymal lumen and associated with spermatozoa. This luminal staining persisted throughout the distal caput, corpus and cauda epididymidis. Staining was rarely associated with spermatozoa in the efferent ducts and initial segment. Alpha-lactalbumin immunoreactivity was also detected in the seminiferous epithelium. Staining was confined to the Golgi-acrosome region of spermatids. These results indicate that an alpha-lactalbumin-like molecule, or molecules, is present in the male reproductive tract and that it is localized specifically in principal cells from the proximal caput epididymidis and germ cells from the seminiferous epithelium.
本文描述了乳蛋白α-乳白蛋白在雄性生殖道中的免疫细胞化学定位。使用针对高度纯化的大鼠乳α-乳白蛋白产生的一抗血清，在附睾头近端主细胞的核上高尔基体区域始终显示出特异性染色，但仅偶尔在管道其他区域的上皮细胞中出现。在附睾管腔中也发现有染色，且与精子有关。这种管腔染色在附睾头远端、附睾体和附睾尾中持续存在。在输出小管和起始段中，染色很少与精子相关。在生精上皮中也检测到了α-乳白蛋白免疫反应性。染色局限于精子细胞的高尔基体-顶体区域。这些结果表明，雄性生殖道中存在一种或多种α-乳白蛋白样分子，并且它特异性地定位于附睾头近端的主细胞和生精上皮的生殖细胞中。
