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印度淡水主要鲤鱼(印度野鲮,Labeo rohita (Hamilton))消化道黏液细胞中的硫黏液素和唾液酸黏液素:一项荧光显微镜研究

Sulfomucins and sialomucins in the mucus cells of the alimentary canal in an Indian freshwater major carp, Labeo rohita (Hamilton): a fluorescence microscopic study.

作者信息

Chakrabarty P, Mukhopadhyay S, Sinha G M

出版信息

Folia Histochem Cytochem (Krakow). 1983;21(3-4):181-6.

PMID:6199266
Abstract

The localization of sulfomucins and sialomucins present in the various types of mucus cells--secretory (SMC) and mature non-secretory mucus cells (MMC) of the different regions of the alimentary canal in Labeo rohita (Ham.) has been studied by fluorescence microscopical techniques (viz., Ferric alum Coriphosphine--FAC, Coriphosphine thiazol yellow--CTY and Dische reaction--DR). High degree of fluorescence activity confirms the presence of relatively high amount of sulfomucins in the mucus cells of the oesophageal and buccopharyngeal regions when tested with FAC and CTY methods while moderate intensity of the same in the mucus cells of the two aforesaid regions indicates the localization of relatively lesser amount of sialomucins studied by DR technique. Moderate degree of fluorescence is also observed in the mucus cells (in both SMC and MMC) of the intestinal region with the techniques of FAC, CTY and DR whereas weak fluorescence of FAC, CTY and DR methods is marked in the mucus cells (in both SMC and MMC) of the rectal region due to the presence of low amount of sulfomucins and sialomucins.

摘要

采用荧光显微镜技术(即,铁明矾考里磷光素——FAC、考里磷光素噻唑黄——CTY和迪施反应——DR)研究了印度野鲮(Ham.)消化道不同区域各种类型黏液细胞——分泌性(SMC)和成熟非分泌性黏液细胞(MMC)中磺黏蛋白和涎黏蛋白的定位。当用FAC和CTY方法检测时,高度的荧光活性证实食管和颊咽区域的黏液细胞中存在相对大量的磺黏蛋白,而用DR技术研究时,上述两个区域的黏液细胞中相同物质的中等强度荧光表明涎黏蛋白的定位相对较少。用FAC、CTY和DR技术在肠道区域的黏液细胞(SMC和MMC中)也观察到中等程度的荧光,而由于磺黏蛋白和涎黏蛋白含量低,在直肠区域的黏液细胞(SMC和MMC中),FAC、CTY和DR方法的荧光较弱。

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