从大肠杆菌核糖核酸酶III突变体中分离出的50S核糖体亚基的异常rRNA连接复合物。
Unusual rRNA-linked complex of 50S ribosomal subunits isolated from an Escherichia coli RNase III mutant.
作者信息
Clark M W, Lake J A
出版信息
J Bacteriol. 1984 Mar;157(3):971-4. doi: 10.1128/jb.157.3.971-974.1984.
Abstract
We have isolated and characterized complexes of ribosomal subunits from Escherichia coli mutant AB301-105 connected by strands of unprocessed RNA. By electron microscopy of these complexes, the location of the 5' end of 5S RNA was established and the location of the 3' end of 23S RNA was confirmed. We also note that in these complexes insertion of 5S rRNA can proceed without the 23S-5S spacer having been processed.
摘要
我们已经分离并鉴定了来自大肠杆菌突变体AB301-105的核糖体亚基复合物，这些复合物由未加工的RNA链连接。通过对这些复合物进行电子显微镜观察，确定了5S RNA 5'端的位置，并证实了23S RNA 3'端的位置。我们还注意到，在这些复合物中，5S rRNA的插入可以在23S-5S间隔区未被加工的情况下进行。
相似文献
1
Unusual rRNA-linked complex of 50S ribosomal subunits isolated from an Escherichia coli RNase III mutant.
J Bacteriol. 1984 Mar;157(3):971-4. doi: 10.1128/jb.157.3.971-974.1984.
2
RNase III cleavage is obligate for maturation but not for function of Escherichia coli pre-23S rRNA.
Proc Natl Acad Sci U S A. 1984 Jan;81(1):185-8. doi: 10.1073/pnas.81.1.185.
3
Structural analysis of 5S rRNA, 5S rRNA-protein complexes and ribosomes employing RNase H and d(GTTCGG).
Eur J Biochem. 1987 Mar 2;163(2):239-46. doi: 10.1111/j.1432-1033.1987.tb10793.x.
4
A mutation in an Escherichia coli ribosomal RNA operon that blocks the production of precursor 23 S ribosomal RNA by RNase III in vivo and in vitro.
J Mol Biol. 1985 Mar 20;182(2):205-16. doi: 10.1016/0022-2836(85)90339-0.
5
Effects of base change mutations within an Escherichia coli ribosomal RNA leader region on rRNA maturation and ribosome formation.
Nucleic Acids Res. 2001 Aug 15;29(16):3394-403. doi: 10.1093/nar/29.16.3394.
6
Characterization of a separate small domain derived from the 5' end of 23S rRNA of an alpha-proteobacterium.
Nucleic Acids Res. 1999 Nov 1;27(21):4241-50. doi: 10.1093/nar/27.21.4241.
7
RNase G (CafA protein) and RNase E are both required for the 5' maturation of 16S ribosomal RNA.
EMBO J. 1999 May 17;18(10):2878-85. doi: 10.1093/emboj/18.10.2878.
8
Functional interaction between RNase III and the Escherichia coli ribosome.
BMC Mol Biol. 2003 Jun 18;4:8. doi: 10.1186/1471-2199-4-8.
9
A ribonucleoprotein precursor of both the 30S and 50S ribosomal sunbunits of Escherichia coli.
Proc Natl Acad Sci U S A. 1975 Apr;72(4):1533-7. doi: 10.1073/pnas.72.4.1533.
10
Crosslinking of 4.5S RNA to the Escherichia coli ribosome in the presence or absence of the protein Ffh.
RNA. 2002 May;8(5):612-25. doi: 10.1017/s1355838202020095.
引用本文的文献
1
Loop IV of 5S ribosomal RNA has contacts both to domain II and to domain V of the 23S RNA.
RNA. 1996 Feb;2(2):146-52.
2
Stem-loop IV of 5S rRNA lies close to the peptidyltransferase center.
Proc Natl Acad Sci U S A. 1994 May 10;91(10):4125-9. doi: 10.1073/pnas.91.10.4125.
3
Structure and function of ribosomal RNA.
Biochem J. 1985 Jul 1;229(1):1-17. doi: 10.1042/bj2290001.
4
DNA hybridization electron microscopy: ribosomal RNA nucleotides 1392-1407 are exposed in the cleft of the small subunit.
Proc Natl Acad Sci U S A. 1986 Jan;83(2):275-9. doi: 10.1073/pnas.83.2.275.
5
RNase III stimulates the translation of the cIII gene of bacteriophage lambda.
Proc Natl Acad Sci U S A. 1987 Sep;84(18):6511-5. doi: 10.1073/pnas.84.18.6511.
6
Nucleolytic processing of ribonucleic acid transcripts in procaryotes.
Microbiol Rev. 1986 Dec;50(4):428-51. doi: 10.1128/mr.50.4.428-451.1986.
本文引用的文献
1
Localization of 3' ends of 5S and 23S rRNAs in reconstituted subunits of Escherichia coli ribosomes.
Proc Natl Acad Sci U S A. 1981 Sep;78(9):5538-42. doi: 10.1073/pnas.78.9.5538.
2
Topography of RNA in the ribosome: localization of the 3'-end of the 23 S rna on the surface of the 50 S ribosomal subunit by immune electron microscopy.
FEBS Lett. 1980 Dec 29;122(2):251-5. doi: 10.1016/0014-5793(80)80450-9.
3
Topography of RNA in the ribosome: location of the 3'-end of 5 S RNA on the central protuberance of the 50 S subunit.
FEBS Lett. 1980 Nov 17;121(1):97-100. doi: 10.1016/0014-5793(80)81274-9.
4
Processing of procaryotic ribonucleic acid.
Microbiol Rev. 1981 Dec;45(4):502-41. doi: 10.1128/mr.45.4.502-541.1981.
5
The ribonuclease III site flanking 23S sequences in the 30S ribosomal precursor RNA of E. coli.
Cell. 1980 Feb;19(2):393-401. doi: 10.1016/0092-8674(80)90513-9.
6
On the catalytic center of peptidyl transfer: a part of the 50 S ribosome structure.
Cold Spring Harb Symp Quant Biol. 1969;34:39-48. doi: 10.1101/sqb.1969.034.01.008.
7
Binding of ribosomal proteins to 30S preribosomal ribonucleic acid of Escherichia coli.
Biochemistry. 1974 Oct 8;13(21):4272-8. doi: 10.1021/bi00718a005.
8
Accumulation of 30S preribosomal ribonucleic acid in an Escherichia coli mutant treated with chloramphenicol.
Biochemistry. 1974 Oct 8;13(21):4268-71. doi: 10.1021/bi00718a004.
9
Alteration in the acetylation level of ribosomal protein L12 during growth cycle of Escherichia coli.
Proc Natl Acad Sci U S A. 1974 May;71(5):2136-40. doi: 10.1073/pnas.71.5.2136.
10
Properties of 30S ribosomal particles reconstituted from precursor 16S ribonucleic acid.
Biochemistry. 1974 Mar 12;13(6):1215-21. doi: 10.1021/bi00703a025.
Zotero 插件