Suyama M, Saito T, Hara K, Kito H, Hosoda S
J Natl Cancer Inst. 1984 Mar;72(3):751-7.
Two biochemically distinguishable transplantable tumor strains (A and B) were established from a primary gastric carcinoid of Mastomys secreting histamine alone. Strain A in the third generation acquired a new ability to produce serotonin [5-hydroxytryptamine (5-HT)] and retained endocrine activities to produce both histamine and 5-HT through the following subpassages, whereas strain B (like the primary tumor) continued to produce histamine alone. The findings were further supported by the immunohistochemical demonstration of 5-HT-containing tumor cells in strain A after generation 3 and the absence of such cells in strain B and also by the ultrastructural demonstration of tumor cells containing pleomorphic secretory granules in strain A after the third generation but not in strain B. Sixteen samples of Formalin-fixed, paraffin-embedded primary gastric carcinoids of Mastomys were stained by the same immunohistochemical method for 5-HT detection. The positively stained tumor cells were demonstrated in 4 tumor samples, though they were scantily distributed in tumor parenchyma except for 1 metastasizing tumor. 5-HT-producing tumor cells appeared through many proliferative cycles of the deranged histamine-producing cells. The endocrinologic similarity was noted between this transplantable tumor strain and a specific type of gastric carcinoid in humans, and the possible histogenesis of the latter tumor was discussed on the basis of data obtained from the present transplantation experiments.
从仅分泌组胺的非洲巨鼠原发性胃类癌中建立了两种生化特性可区分的可移植肿瘤株（A和B）。第三代的A株获得了产生5-羟色胺[5-羟色胺（5-HT）]的新能力，并在随后的传代过程中保留了产生组胺和5-HT的内分泌活性，而B株（与原发性肿瘤一样）继续仅产生组胺。第三代后A株中含5-HT肿瘤细胞的免疫组化显示以及B株中不存在此类细胞，以及第三代后A株中含多形性分泌颗粒的肿瘤细胞的超微结构显示而B株中未显示，进一步支持了这些发现。对16份福尔马林固定、石蜡包埋的非洲巨鼠原发性胃类癌样本采用相同的免疫组化方法进行5-HT检测染色。在4个肿瘤样本中发现了阳性染色的肿瘤细胞，不过除了1个转移瘤外，它们在肿瘤实质中的分布很少。产生5-HT的肿瘤细胞是通过产生组胺的紊乱细胞的许多增殖周期出现的。注意到这种可移植肿瘤株与人类一种特定类型的胃类癌之间存在内分泌学相似性，并根据目前移植实验获得的数据讨论了后者肿瘤可能的组织发生。
