Zehender H, Trescher D, Ullrich J
Anal Biochem. 1983 Nov;135(1):16-21. doi: 10.1016/0003-2697(83)90724-8.
A method for the localization of pyruvate decarboxylase bands in polyacrylamide gels after electrophoresis using 1,2-dianilinoethane in dilute acetic acid as reagent for acetaldehyde formed from pyruvate by the holoenzyme is described. A white condensation product forms in the bands and precipitates within a few minutes. The more or less opaque bands can be viewed and photographed against a dark background and scanned in a densitometer. The detection limit is at about 10 mU pyruvate decarboxylase when the gels are bathed in the staining solution for 20 min. Several other methods were tested and failed to produce satisfactory results.
描述了一种电泳后在聚丙烯酰胺凝胶中定位丙酮酸脱羧酶条带的方法，该方法使用稀乙酸中的1,2 - 二苯胺乙烷作为试剂，用于检测全酶由丙酮酸形成的乙醛。在条带中形成白色缩合产物，并在几分钟内沉淀。或多或少不透明的条带可以在深色背景下观察和拍照，并在密度计中进行扫描。当凝胶在染色溶液中浸泡20分钟时，检测限约为10 mU丙酮酸脱羧酶。还测试了其他几种方法，但均未产生满意的结果。
