在Triton-酸-尿素凝胶中对组蛋白进行银染色。
Silver staining of histones in Triton-acid-urea gels.
作者信息
Mold D E, Weingart J, Assaraf J, Lubahn D B, Kelner D N, Shaw B R, McCarty K S
出版信息
Anal Biochem. 1983 Nov;135(1):44-7. doi: 10.1016/0003-2697(83)90727-3.
A reliable method for silver staining histones in Triton-acid-urea gels was developed. Optimum staining is achieved by treating the gels either with amido black or a colorless, water-soluble analog of amido black, 2,7-naphthalenedisulfonic acid, prior to staining with ammoniacal silver. Staining of purified calf thymus histones H2A, H2B, H3, and H4 by this method is 30 times more sensitive than staining with amido black alone, allowing the detection of each histone and its modified forms down to the nanogram level. The use of 2,7-naphthalenedisulfonic acid dramatically shortens the procedure permitting histone patterns to be visualized within 5 h.
开发了一种在Triton-酸-尿素凝胶中对组蛋白进行银染的可靠方法。在用氨性银染色之前，用酰胺黑或酰胺黑的无色水溶性类似物2,7-萘二磺酸处理凝胶，可实现最佳染色效果。用这种方法对纯化的小牛胸腺组蛋白H2A、H2B、H3和H4进行染色，其灵敏度比单独使用酰胺黑高30倍，能够检测到每种组蛋白及其修饰形式，最低可达纳克水平。使用2,7-萘二磺酸可显著缩短染色过程，使组蛋白条带在5小时内即可显现。
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