Nkrumah F K, Neequaye J, Twum-Danso K
Ann Trop Paediatr. 1983 Dec;3(4):193-6. doi: 10.1080/02724936.1983.11748293.
Gram-stained buffy-coat smears from venous and capillary blood samples in 105 children suspected of septicaemia were examined for the presence of bacteria and the results compared with blood culture isolates. Gram-positive and Gram-negative bacteria were identified in 18 venous (44%) and 19 capillary (46%) buffy-coat preparations in 41 instances where bacterial organisms were isolated from the blood cultures. It is concluded that the examination of buffy-coat smears for bacteria in children suspected of septicaemia is a useful adjunct to blood cultures and, in areas where no facilities exist for culture of blood, may be a simple and rapid method of establishing the diagnosis of bacteraemia in suspected patients.
对105名疑似败血症儿童的静脉血和毛细血管血样本进行革兰氏染色的血沉棕黄层涂片检查，以检测细菌的存在，并将结果与血培养分离株进行比较。在从血培养中分离出细菌的41例病例中，在18份静脉血沉棕黄层涂片（44%）和19份毛细血管血沉棕黄层涂片（46%）中鉴定出革兰氏阳性菌和革兰氏阴性菌。结论是，对疑似败血症儿童的血沉棕黄层涂片进行细菌检查是血培养的一项有用辅助检查，在没有血液培养设施的地区，可能是一种简单快速的方法，用于对疑似患者进行菌血症诊断。
