在牛和羊的垂体、牛脑及肾上腺髓质中检测牛胰蛋白酶抑制剂（bPTI）。一种针对bPTI的特异性放射免疫测定法。
Detection of bovine pancreatic trypsin inhibitor (bPTI) in bovine and ovine pituitaries, bovine brain, and adrenal medulla. A specific radioimmunoassay for bPTI.
作者信息
Westphal M, Hammonds R G, Li C H
出版信息
Arch Biochem Biophys. 1984 Mar;229(2):555-9. doi: 10.1016/0003-9861(84)90187-5.
An antiserum against bovine pancreatic trypsin inhibitor (bPTI) has been raised in rabbits. The antiserum does not cross-react with known pituitary hormones and other proteins. A specific radioimmunoassay for bPTI has been developed. The ED50 is about 250 fmol per assay tube and the sensitivity is reliable to 15 fmol per tube. Immunoreactivity could be detected in bovine and sheep pituitaries, bovine brain, and adrenal medulla, but not in human, pig, and rat pituitaries. In the bovine pituitary the immunoreactivity is restricted to the posterior lobe.
已经在兔子体内产生了一种针对牛胰蛋白酶抑制剂（bPTI）的抗血清。该抗血清与已知的垂体激素和其他蛋白质不发生交叉反应。已经开发出一种针对bPTI的特异性放射免疫测定法。半数有效剂量（ED50）约为每个测定管250飞摩尔，灵敏度可靠至每个管15飞摩尔。在牛和羊的垂体、牛脑和肾上腺髓质中可检测到免疫反应性，但在人、猪和大鼠的垂体中未检测到。在牛垂体中，免疫反应性仅限于后叶。
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