Antigenic hemagglutination inhibition test in examining of the culture supernatants of Clostridium botulinum types A, B, E, and F and some related bacterial species.

The hemagglutination inhibition test with using lyophilized preparations of the double botulinal indicator system (antigen sensitized erythrocytes and type-specific homologous antitoxic globulin) was used to the identification of the Cl. botulinum types A, B, E and F culture supernatants and the supernatants of other related bacteria. In the examined indicator hemagglutination system, the type-specific results were obtained in spite of the existence of antigenic cross-reactivity known inside the genus Clostridium. Absence hemagglutination inhibition reaction of the botulinal indicator system with cultured supernatants of the protein A - positive Staph. aureus strains was discussed.