大鼠肝脏线粒体天冬氨酸氨基转移酶信使核糖核酸：游离多核糖体合成更高分子量的前体。

Rat liver mitochondrial aspartate aminotransferase mRNA: synthesis of an higher molecular weight precursor by free polysomes.

作者信息

Marra E, Greco M, Quagliariello E, Saccone C

出版信息

Boll Soc Ital Biol Sper. 1983 Dec 30;59(12):1785-90.

Abstract

The site of synthesis of the higher molecular weight precursor of mAAT has been determined by separation of free and membrane-bound polysomes. Free and bound polysomes were isolated from rat liver and the polyA+RNA was extracted. Protein synthesis was carried out for two hours by using a reticulocyte lysate system in the presence of 35S-methionine. The samples were subjected to immunoprecipitation with specific antiserum. The precipitates were analyzed by Na-DodSO4/polyacrylamide gel electrophoresis and fluorography. The results clearly demonstrate that the enzyme is synthesized by free polysomes.

摘要

通过分离游离和膜结合多核糖体，已确定了高分子量前体 mAAT 的合成位点。从大鼠肝脏中分离出游离和结合多核糖体，并提取了 polyA+RNA。在 35S-甲硫氨酸存在的情况下，使用网织红细胞裂解物系统进行了两小时的蛋白质合成。样品用特异性抗血清进行免疫沉淀。沉淀物通过 Na-DodSO4/聚丙烯酰胺凝胶电泳和荧光自显影进行分析。结果清楚地表明该酶是由游离多核糖体合成的。

相似文献

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Rat liver mitochondrial aspartate aminotransferase mRNA: synthesis of an higher molecular weight precursor by free polysomes.大鼠肝脏线粒体天冬氨酸氨基转移酶信使核糖核酸：游离多核糖体合成更高分子量的前体。

Boll Soc Ital Biol Sper. 1983 Dec 30;59(12):1785-90.

2

Rat liver mitochondrial aspartate aminotransferase mRNA: isolation of mRNA by polyacrylamide gel electrophoresis.

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