Pobor G, Pettersson S, Bandeira A, Martinez-A C, Coutinho A
Eur J Immunol. 1984 Mar;14(3):222-7. doi: 10.1002/eji.1830140305.
This study has investigated whether exclusive recognition of I-A or I-E molecules on the B cell surface by T helper cells is sufficient to activate resting B cells. Lines and clones of long-term-cultured T helper cells with specificity for I-A or I-E antigens have been derived from mixed lymphocyte cultures between spleen cells from major histocompatibility complex (MHC)-congenic mouse strains. These cells were tested for helper activity and proved competent to induce resting B lymphocytes expressing the specific MHC antigens to polyclonal expansion and maturation to Ig secretion. B cell activation was shown to require direct recognition of I-A/E antigens by the helper cells on the responding B lymphocyte surface and it could not be achieved by soluble factors released by "third-party" helper cell activity ongoing in the same cultures. Since B lymphocyte activation occurs in the absence of antigen recognition by the responding B cells, these observations suggest that I-A and I-E molecules expressed on the B cell surface participate in the functional reception of T helper cell-derived induction signals.
本研究调查了辅助性T细胞对B细胞表面I - A或I - E分子的特异性识别是否足以激活静息B细胞。对I - A或I - E抗原有特异性的长期培养辅助性T细胞系和克隆是从主要组织相容性复合体（MHC）同基因小鼠品系的脾细胞之间的混合淋巴细胞培养物中获得的。检测了这些细胞的辅助活性，结果证明它们有能力诱导表达特异性MHC抗原的静息B淋巴细胞进行多克隆扩增并成熟至分泌免疫球蛋白。研究表明，B细胞激活需要辅助性T细胞直接识别应答B淋巴细胞表面的I - A/E抗原，而同一培养物中“第三方”辅助性T细胞活动释放的可溶性因子无法实现这一点。由于B淋巴细胞激活发生在应答B细胞不识别抗原的情况下，这些观察结果表明，B细胞表面表达的I - A和I - E分子参与了辅助性T细胞衍生诱导信号的功能性接收。
