一种以刚果/克里米亚出血热病毒为模型增加树脂包埋病毒表面细节的方法。
A method for increasing the surface details of resin embedded viruses using Congo/Crimean haemorrhagic fever virus as a model.
作者信息
Ellis D S, Smith M D, Lloyd G, Bowen E T, Simpson D I
出版信息
J Virol Methods. 1984 Feb;8(1-2):57-61. doi: 10.1016/0166-0934(84)90040-5.
PMID:6200490
Abstract
The surface details of resin embedded viruses can be greatly enhanced by the use of uranyl acetate block staining. The virions are embedded in agar immediately after fixation and block stained with 3% uranyl acetate in methanol during dehydration. This technique allows those particles outside their host cells to show clearly the spikes normally seen only by the separate use of negative staining.
摘要
通过使用醋酸铀块染色，可极大地增强树脂包埋病毒的表面细节。病毒粒子在固定后立即嵌入琼脂中，并在脱水过程中用3%醋酸铀甲醇溶液进行块染色。这项技术使那些处于宿主细胞外的颗粒能够清晰显示出通常只有通过单独使用负染色才能看到的刺突。
相似文献
1
A method for increasing the surface details of resin embedded viruses using Congo/Crimean haemorrhagic fever virus as a model.一种以刚果/克里米亚出血热病毒为模型增加树脂包埋病毒表面细节的方法。
J Virol Methods. 1984 Feb;8(1-2):57-61. doi: 10.1016/0166-0934(84)90040-5.
2
Congo/Crimean haemorrhagic fever virus from Iraq 1979: I. Morphology in BHK21 cells.1979年来自伊拉克的刚果/克里米亚出血热病毒：I. 在BHK21细胞中的形态学
Arch Virol. 1981;70(3):189-98. doi: 10.1007/BF01315125.
3
[Characteristics of Xinjiang hemorrhagic fever virus. III. Electron microscopic observation of Xinjiang hemorrhagic fever virus].[新疆出血热病毒的特性。III. 新疆出血热病毒的电子显微镜观察]
Zhonghua Liu Xing Bing Xue Za Zhi. 1983 Jun;4(3):135-6.
4
Crimean-Congo haemorrhagic fever virus in Iraq: isolation, identification and electron microscopy.伊拉克的克里米亚-刚果出血热病毒：分离、鉴定及电子显微镜观察
Acta Virol. 1980 Dec;24(6):464-7.
5
Physicochemical characteristics, morphology and morphogenesis of virions of the causative agent of Crimean hemorrhagic fever.
Intervirology. 1977;8(5):294-308. doi: 10.1159/000148904.
6
Morphology and morphogenesis of Crimean hemorrhagic fever virus.克里米亚出血热病毒的形态学与形态发生
Arch Virol. 1976;50(1-2):169-72. doi: 10.1007/BF01318011.
7
Hantaan virus, aetiological agent of Korean haemorrhagic fever, has Bunyaviridae-like morphology.汉坦病毒是韩国出血热的病原体，具有类似布尼亚病毒科的形态。
Lancet. 1982 Apr 3;1(8275):768-71. doi: 10.1016/s0140-6736(82)91813-x.
8
Isolation of Crimean-Congo haemorrhagic fever and Rift Valley fever viruses in Upper Volta.在沃尔特河上游地区分离出克里米亚-刚果出血热病毒和裂谷热病毒。
Lancet. 1984 May 26;1(8387):1179. doi: 10.1016/s0140-6736(84)91419-3.
9
Ex vivo stability of the rodent-borne Hantaan virus in comparison to that of arthropod-borne members of the Bunyaviridae family.与布尼亚病毒科节肢动物传播成员相比，啮齿动物传播的汉坦病毒的体外稳定性。
Appl Environ Microbiol. 2007 Apr;73(8):2547-51. doi: 10.1128/AEM.02869-06. Epub 2007 Mar 2.
10
Electron microscopic demonstration of Crimean hemorrhagic fever virus in CV-1 cells.
Acta Virol. 1975 Sep;19(5):369-73.
Zotero 插件