一种以刚果/克里米亚出血热病毒为模型增加树脂包埋病毒表面细节的方法。
A method for increasing the surface details of resin embedded viruses using Congo/Crimean haemorrhagic fever virus as a model.
作者信息
Ellis D S, Smith M D, Lloyd G, Bowen E T, Simpson D I
出版信息
J Virol Methods. 1984 Feb;8(1-2):57-61. doi: 10.1016/0166-0934(84)90040-5.
PMID:6200490
Abstract
The surface details of resin embedded viruses can be greatly enhanced by the use of uranyl acetate block staining. The virions are embedded in agar immediately after fixation and block stained with 3% uranyl acetate in methanol during dehydration. This technique allows those particles outside their host cells to show clearly the spikes normally seen only by the separate use of negative staining.
摘要
通过使用醋酸铀块染色，可极大地增强树脂包埋病毒的表面细节。病毒粒子在固定后立即嵌入琼脂中，并在脱水过程中用3%醋酸铀甲醇溶液进行块染色。这项技术使那些处于宿主细胞外的颗粒能够清晰显示出通常只有通过单独使用负染色才能看到的刺突。
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