Barakat I, Sensenbrenner M, Labourdette G
J Neurosci Res. 1984;11(2):117-29. doi: 10.1002/jnr.490110202.
Extract was prepared from bovine brain by treatment with chloroform-methanol, drying of the precipitate, and successive extractions of the powder with Tris buffer. Influence of this extract on neuroblast proliferation in culture was tested. Neuroblast cultures were prepared from brain hemispheres of 6-day-old chick embryos. Cell proliferation was quantified by incorporation of 3H-thymidine. The brain extract was able to stimulate the proliferation of the neuroblasts in culture. In order to purify the factor responsible of this effect, the brain extract was subjected to diethylaminoethyl (DEAE)-cellulose chromatography and gel filtrations. By UV spectrography and destruction of the activity with RNAase and KOH, it was shown that in the extract RNAs are responsible for at least a part of the effect on the proliferation of neuroblasts in culture. Brain RNA is not specific for this effect, since mouse ribosomal RNA was also active. The effect could be actually elicited by some nucleotides.
通过用氯仿 - 甲醇处理牛脑、干燥沉淀，并将粉末依次用Tris缓冲液萃取来制备提取物。测试了该提取物对培养中神经母细胞增殖的影响。神经母细胞培养物取自6日龄鸡胚的脑半球。通过掺入3H - 胸腺嘧啶核苷来定量细胞增殖。脑提取物能够刺激培养中的神经母细胞增殖。为了纯化造成这种效应的因子，对脑提取物进行了二乙氨基乙基（DEAE） - 纤维素色谱和凝胶过滤。通过紫外光谱法以及用RNA酶和氢氧化钾破坏活性，结果表明提取物中的RNA至少部分负责对培养中神经母细胞增殖的影响。脑RNA对这种效应不具有特异性，因为小鼠核糖体RNA也具有活性。实际上某些核苷酸可以引发这种效应。
