Donnelly B J, Lakey W H, McBlain W A
J Urol. 1984 Apr;131(4):806-11. doi: 10.1016/s0022-5347(17)50629-1.
To further characterize human prostatic androgen receptor, nuclei were isolated from normal prostate (no. = 3) and benign prostatic hyperplasia specimens (no. = 10). High ionic strength (0.6 M KCl) treatment of nuclei released nuclear extractable androgen receptor and DNase I digestion then yielded nuclear matrices. Androgen receptor was quantified in the nuclear extract and nuclear matrix preparations by Scatchard analysis of specific R1881 binding. Only 1 of the 3 normal tissues had extractable androgen receptor (113 fmol. per gm. of tissue) while the mean concentration of extractable androgen receptor for BPH was 189 fmol. per gm. of tissue. The mean concentrations of matrix-bound androgen receptor were 325 fmol. per gm. of tissue and 548 fmol. per gm. of tissue for normal and hyperplastic prostate, respectively. The androgen binding sites on nuclear matrix may represent the functional intranuclear androgen receptor and a characterization of these sites may provide an understanding of the etiology of BPH.
为了进一步表征人前列腺雄激素受体，从正常前列腺（n = 3）和良性前列腺增生标本（n = 10）中分离出细胞核。用高离子强度（0.6M KCl）处理细胞核，释放出可提取的核雄激素受体，然后用DNase I消化得到核基质。通过对特异性R1881结合的Scatchard分析，对核提取物和核基质制剂中的雄激素受体进行定量。3个正常组织中只有1个具有可提取的雄激素受体（每克组织113 fmol），而良性前列腺增生的可提取雄激素受体平均浓度为每克组织189 fmol。正常前列腺和增生性前列腺的基质结合雄激素受体平均浓度分别为每克组织325 fmol和每克组织548 fmol。核基质上的雄激素结合位点可能代表功能性核内雄激素受体，对这些位点的表征可能有助于了解良性前列腺增生的病因。
