Chappard D, Alexandre C, Camps M, Montheard J P, Riffat G
Stain Technol. 1983 Sep;58(5):299-308. doi: 10.3109/10520298309066802.
A mixture of pure and anhydrous glycol methacrylate and methyl methacrylate is used as an embedding medium for iliac bone biopsies. Infiltration is carried out at -20 C with the embedding medium and a cold inactivated catalyst-initiator system. Raising the temperature to 4 C initiates polymerization and limits the peak temperature of polymerization to 25 C. In this way, such thermolabile enzymes as osteoclastic acid phosphatase are preserved. After staining, sections are dehydrated in polyethylene glycol 400 30% in 2-propanol. This gives flat sections and improves staining properties.
纯无水甲基丙烯酸乙二醇酯和甲基丙烯酸甲酯的混合物用作髂骨活检的包埋介质。在-20℃下用包埋介质和冷灭活的催化剂-引发剂系统进行渗透。将温度升至4℃引发聚合反应，并将聚合反应的峰值温度限制在25℃。通过这种方式，诸如破骨细胞酸性磷酸酶等热不稳定酶得以保存。染色后，切片在含30%聚乙二醇400的异丙醇中脱水。这样可得到平整的切片并改善染色特性。
