一种用于低温甲基丙烯酸甲酯包埋的简化技术。

A simplified technique for low temperature methyl methacrylate embedding.

作者信息

Liu C C

出版信息

Stain Technol. 1987 May;62(3):155-9. doi: 10.3109/10520298709107986.

DOI:10.3109/10520298709107986

Abstract

A simplified method for low temperature methyl methacrylate embedding with inhibited methyl methacrylate monomer is demonstrated using proper concentrations of benzoyl peroxide and N,N-dimethylaniline. The polymerized tissue blocks cut well and the tissue sections obtained show excellent acid phosphatase activity when demonstrated with the newly improved technique and Goldner's staining. Likewise, double tetracycline labels are well revealed by fluorescence microscopy.

摘要

本文展示了一种使用适当浓度的过氧化苯甲酰和N，N-二甲基苯胺，以抑制甲基丙烯酸甲酯单体进行低温甲基丙烯酸甲酯包埋的简化方法。聚合后的组织块易于切割，并且当采用新改进的技术和戈德纳染色法进行检测时，所获得的组织切片显示出优异的酸性磷酸酶活性。同样，荧光显微镜能很好地显示双四环素标记。