Degtiar' V G, Miloserdov Iu V, Kushlinskiĭ N E
Vopr Med Khim. 1984 Jan-Feb;30(1):122-6.
A simple procedure is developed for estimation of proteins after precipitation with protamine sulfate in cytosol fraction of human tissues-target dor sex steroids (prostate adenoma, cancer of prostate, mammalian gland cancer) and in similar target tissues of rats (ventral prostate and uterus). Human blood serum did not interfere with the protein estimation after the protamine sulfate precipitation. This method for estimation of protein using protamine sulfate might be critically important in calculation of the binding capacity of target-tissues with sex steroids (quantitative estimation of receptors), especially in human tissues because the interference of blood proteins is eliminated. The efficiency of the procedure developed was confirmed by comparative study of binding capacity of the tissues of prostate adenoma, cancer of prostate and mammalian gland cancer towards 3H-17 beta-estradiol and 3H-dihydrotestosterone, calculated per total protein and the protein precipitated by protamine sulfate.
开发了一种简单的程序，用于在人组织（目标为性类固醇，如前列腺腺瘤、前列腺癌、乳腺癌）的胞质溶胶部分以及大鼠的类似目标组织（腹侧前列腺和子宫）中，用硫酸鱼精蛋白沉淀后估计蛋白质含量。硫酸鱼精蛋白沉淀后，人血清不干扰蛋白质估计。这种使用硫酸鱼精蛋白估计蛋白质的方法，对于计算目标组织与性类固醇的结合能力（受体的定量估计）可能至关重要，特别是在人体组织中，因为消除了血液蛋白质的干扰。通过比较前列腺腺瘤、前列腺癌和乳腺癌组织对3H-17β-雌二醇和3H-双氢睾酮的结合能力（按总蛋白和硫酸鱼精蛋白沉淀的蛋白计算），证实了所开发程序的有效性。
