Bell P A, Jones T R, Weatherill P J
Biochem Biophys Res Commun. 1984 Mar 30;119(3):1134-40. doi: 10.1016/0006-291x(84)90893-3.
Dissociation kinetics were determined at 0 degrees C for molybdate-stabilized glucocorticoid-receptor complexes in rat thymus cytosol. Exposure of complexes to dextran-coated charcoal had no effect on their chromatographic properties or transformation status, but dissociation rates measured after charcoal treatment were significantly lower than those determined by displacement with excess competing steroid. The dissociation rate of the [2,4,6,7-3H]prednisolone-receptor complex was similarly modified by chromatography on Lipidex 1000, but not by chromatography on Sephadex G-25 or G-75. It is concluded that treatment of glucocorticoid-receptor complexes with dextran-charcoal or Lipidex 1000 brings about a change in dissociation rate as a consequence of the removal of a lipid component from the complex.
在0摄氏度下测定了大鼠胸腺细胞质中钼酸盐稳定的糖皮质激素受体复合物的解离动力学。将复合物暴露于葡聚糖包被的活性炭对其色谱性质或转化状态没有影响，但经活性炭处理后测得的解离速率明显低于用过量竞争性类固醇置换所测定的解离速率。[2,4,6,7-³H]泼尼松龙受体复合物的解离速率同样因在Lipidex 1000上进行色谱分析而改变，但在Sephadex G-25或G-75上进行色谱分析则没有这种情况。得出的结论是，用葡聚糖-活性炭或Lipidex 1000处理糖皮质激素受体复合物会导致解离速率发生变化，这是由于复合物中的脂质成分被去除所致。
