Gupta B B
J Chromatogr. 1983 Dec 30;282:463-75. doi: 10.1016/s0021-9673(00)91623-6.
High-performance size exclusion chromatography on a TSK 3000 SW column was used to separate and quantify the individual milk proteins in their native and/or denatured state. Quantitation of the individual native whey proteins within +/- 10% was achieved. The denatured proteins were dissociated into their monomer units by using sodium dodecyl sulphate and beta-mercaptoethanol. A good separation of the whey protein monomers was achieved and the quantitation of the individual whey proteins within +/- 10% was possible. Under these conditions, alpha s1- and beta-casein were eluted as one peak, whereas kappa-casein and gamma-casein were eluted in the same position as beta-lactoglobulin and alpha-lactalbumin respectively. A method for calculating the areas and hence the concentration of the individual proteins in a mixture of casein and whey proteins is described.
使用TSK 3000 SW柱上的高效尺寸排阻色谱法来分离和定量处于天然和/或变性状态的各个乳蛋白。实现了对各个天然乳清蛋白的定量，误差在±10%以内。通过使用十二烷基硫酸钠和β-巯基乙醇将变性蛋白解离成其单体单元。实现了乳清蛋白单体的良好分离，并且能够在±10%的误差范围内对各个乳清蛋白进行定量。在这些条件下，αs1-酪蛋白和β-酪蛋白作为一个峰被洗脱，而κ-酪蛋白和γ-酪蛋白分别在与β-乳球蛋白和α-乳白蛋白相同的位置被洗脱。描述了一种计算酪蛋白和乳清蛋白混合物中各个蛋白质的面积以及浓度的方法。
