Holder A A, Freeman R R, Newbold C I
Mol Biochem Parasitol. 1983 Nov;9(3):191-6. doi: 10.1016/0166-6851(83)90096-8.
A 230 000 molecular weight (MW) Plasmodium yoelii protein, a 250 000 MW P. chabaudi protein and a 195 000 MW P. falciparum protein, identified using monoclonal antibodies, have similar characteristics, and have been implicated as protective antigens. In this study the serological relationship between these proteins was investigated by Western transfer analysis. The monoclonal antibodies specific for each of the high molecular weight proteins did not cross-react with antigens of the other two parasites, but a polyvalent mouse serum raised against the purified 230 000 MW P. yoelii protein cross-reacted with the high molecular weight proteins of P. chabaudi and P. falciparum and also with the fragments derived from these proteins. This result indicates that these proteins belong to the same class of malaria parasite antigen.
用单克隆抗体鉴定出的分子量为230000的约氏疟原虫蛋白、分子量为250000的查巴迪疟原虫蛋白和分子量为195000的恶性疟原虫蛋白具有相似的特性，并且被认为是保护性抗原。在本研究中，通过蛋白质印迹分析研究了这些蛋白之间的血清学关系。针对每种高分子量蛋白的单克隆抗体不会与其他两种寄生虫的抗原发生交叉反应，但用纯化的分子量为230000的约氏疟原虫蛋白免疫小鼠制备的多价血清会与查巴迪疟原虫和恶性疟原虫的高分子量蛋白以及这些蛋白衍生的片段发生交叉反应。这一结果表明，这些蛋白属于同一类疟原虫抗原。
