Hruby S, Alvord E C, Martenson R E, Deibler G E, Hickey W F, Gonatas N K
Prog Clin Biol Res. 1984;146:271-6.
The epitopes (antigenic determinants) evoked by guinea pig or monkey myelin basic protein (BP) for 7 monoclonal antibodies (MAb) have been localized to 4 different sequences of the BPs extracted from various species. The first pair of epitopes is included within residues 1-14 and reacts with 2 rat IgG MAbs having slightly different specificities. The second pair of epitopes overlap within the sequence 86-100, requiring the Phe(91)-Phe(92) residues. One includes only residues 90-100 and reacts with a rat IgG MAb, the other is longer and reacts with a mouse IgM MAb. The third epitope lies within residues 114-124 (specifically including the single Trp) and reacts with a rat IgG MAb. A cross-reacting epitope in the amino half of the molecule is present in certain species (human, bovine and guinea pig). The fourth pair of epitopes includes residues 131-140 and is severely species-restricted. A mouse IgM MAb reacts practically only with guinea pig BP. Another IgG MAb reacts with human, chimpanzee, monkey, bovine and rat-18.5 kDa BPs, to a much lesser extent with rabbit and rat-14 kDa BPs and not with guinea pig, pig or chicken BPs. A cross-reacting epitope in the amino half of the molecule may be present in the more reactive species.
豚鼠或猴髓鞘碱性蛋白（BP）针对7种单克隆抗体（MAb）所引发的表位（抗原决定簇）已定位到从不同物种提取的BP的4个不同序列上。第一对表位包含在第1 - 14位残基内，并与2种具有略有不同特异性的大鼠IgG MAb发生反应。第二对表位在第86 - 100位序列内重叠，需要苯丙氨酸（91）-苯丙氨酸（92）残基。其中一个仅包含第90 - 100位残基，并与一种大鼠IgG MAb发生反应，另一个更长，并与一种小鼠IgM MAb发生反应。第三个表位位于第114 - 124位残基内（特别包括单个色氨酸），并与一种大鼠IgG MAb发生反应。分子氨基端的一个交叉反应表位存在于某些物种（人类、牛和豚鼠）中。第四对表位包括第131 - 140位残基，并且具有严格的物种限制性。一种小鼠IgM MAb实际上仅与豚鼠BP发生反应。另一种IgG MAb与人类、黑猩猩、猴、牛和大鼠 - 18.5 kDa BP发生反应，与兔和大鼠 - 14 kDa BP的反应程度要小得多，而不与豚鼠、猪或鸡的BP发生反应。分子氨基端的一个交叉反应表位可能存在于反应性更强的物种中。
