Weerasinghe K M, Scully M F, Kakkar V V
Thromb Haemost. 1984 Feb 28;51(1):37-41.
Collagen mediated platelet aggregation caused -5.6 +/- 6.7% inhibition and +39.1 +/- 15.2% potentiation of prekallikrein activation in plasma from normal healthy volunteers between 20-40 and 50-65 years of age, respectively (n = 15, p less than 0.01). The amounts of platelets factor-four (PF4) released in the two groups were not significantly different. Collagen treatment in the presence of indomethacin caused +11.5 +/- 3.6% and +59.6 +/- 19.5% potentiation in the 20-40 and 50-65 age groups respectively (p less than 0.02). Adrenaline mediated platelet aggregation caused -55.2 +/- 7.1% and -35.2 +/- 8.3% inhibition in the 20-40 and 50-65 age groups, respectively. Collagen treatment of platelet-deficient-plasma and platelet-rich-plasma in EDTA also caused potentiation of prekallikrein activation. The results indicate that the observed degree of prekallikrein activation after platelet aggregation is a net result of the inhibitory effect of PF4 and the potentiatory effect of activated platelets. The potentiatory effect was greater after collagen treatment as compared to adrenaline treatment, and in the 50-65 age group as compared to the 20-40 age group.
胶原蛋白介导的血小板聚集分别导致20 - 40岁和50 - 65岁正常健康志愿者血浆中前激肽释放酶激活受到5.6±6.7%的抑制和39.1±15.2%的增强（n = 15，p < 0.01）。两组中释放的血小板因子4（PF4）量无显著差异。在吲哚美辛存在的情况下，胶原蛋白处理在20 - 40岁和50 - 65岁年龄组中分别导致11.5±3.6%和59.6±19.5%的增强（p < 0.02）。肾上腺素介导的血小板聚集在20 - 40岁和50 - 65岁年龄组中分别导致55.2±7.1%和35.2±8.3%的抑制。在EDTA中对血小板缺乏血浆和富含血小板血浆进行胶原蛋白处理也导致前激肽释放酶激活增强。结果表明，血小板聚集后观察到的前激肽释放酶激活程度是PF4的抑制作用和活化血小板的增强作用的净结果。与肾上腺素处理相比，胶原蛋白处理后的增强作用更大，且在50 - 65岁年龄组中比20 - 40岁年龄组更大。
