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红链霉菌质粒的分离与鉴定

Isolation and characterization of Streptomyces erythreus plasmids.

作者信息

Czernik P, Kauc L, Kutner M, Piekarowicz A

出版信息

Acta Microbiol Pol. 1983;32(4):327-37.

PMID:6202100
Abstract

Streptomyces erythreus strains were found to carry several plasmids of molecular weights ranging from about 2 X 10(6) Mr to 40 X 10(6) Mr. Restriction enzyme maps for the streptomycete plasmids pPC7 and pPC8 were constructed for the enzymes Bg/II, EcoRI, XbaI, HindIII, BamHI and SalI. The smaller, pPC8, plasmid appears to be a naturally occurring deletion variant of pPC7. These plasmids belong to the group of conjugative streptomycete plasmids.

摘要

人们发现红色链霉菌菌株携带几种分子量范围约为2×10⁶道尔顿至40×10⁶道尔顿的质粒。构建了链霉菌质粒pPC7和pPC8针对限制性内切酶Bg/II、EcoRI、XbaI、HindIII、BamHI和SalI的限制性酶切图谱。较小的质粒pPC8似乎是pPC7的天然缺失变体。这些质粒属于接合型链霉菌质粒组。

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