[Effect of collagenase, hyaluronidase and pronase, alone or combined with cationic markers, on the renal filtration barrier in the rat].

The authors investigate the effect of collagenase, hyaluronidase and pronase, used either alone or associated with cationic tracers such as Alcian Blue, Ruth enium Red and Polyethyleneimine on the Rat glomerular filtration barrier. Collagenase appears to promote the perviousness of the three constitutive layers of the glomerular basement membrane. The effects of hyaluronidase and pronase seem to be specific and permit to suppose that the glomerular basement membrane supports several types of anionic sites. The affinity of cationic tracers seems to fluctuate according to the constitution of these sites.