Araki M, Watanabe K, Yasuda K
Cell Struct Funct. 1984 Mar;9(1):1-12. doi: 10.1247/csf.9.1.
Photoreceptor cells in chick retina consist of a rod and five types of cones. Immunocytochemical techniques have pinpointed rhodopsin-like immunoreactivity in the outer segments of some single cones as well as in rods. The antibody used in our study was raised against bovine rhodopsin purified by SDS-polyacrylamide gel electrophoresis. No rhodopsin-like immunoreactivity was found in the principal and accessory cone. Epon-embedded, semi-thin sections also were treated with potassium hydroxide to remove epoxy resin and then subjected to immunoreaction with the antibody. With this method the same results were obtained repeatedly, and the possibility of staining failure in double cones and some single cones due to insufficient permeability was avoided. Electron microscopy revealed that the oil droplets of stained single cones are slightly smaller and are located in a more vitreal position than those of unstained single cones. The stained single cones are presumed to be types II and III single cones. In the inner segment, especially in the Golgi region and the rER, no staining was seen. Only slight staining was found in the connecting cilium and in the small vesicular or granular structure between the oil droplet and the plasma membrane facing the heavily stained outer segment. This suggests that opsin is incorporated into the plasma membrane in the distal portion of the inner segment.
鸡视网膜中的光感受器细胞由一根视杆和五种视锥细胞组成。免疫细胞化学技术已确定，在一些单个视锥细胞的外段以及视杆中存在视紫红质样免疫反应性。我们研究中使用的抗体是针对通过SDS-聚丙烯酰胺凝胶电泳纯化的牛视紫红质产生的。在主视锥细胞和副视锥细胞中未发现视紫红质样免疫反应性。用环氧树脂包埋的半薄切片也用氢氧化钾处理以去除环氧树脂，然后与抗体进行免疫反应。用这种方法反复获得相同的结果，避免了由于通透性不足导致双视锥细胞和一些单个视锥细胞染色失败的可能性。电子显微镜显示，染色的单个视锥细胞的油滴比未染色的单个视锥细胞的油滴略小，且位于更靠近玻璃体的位置。染色的单个视锥细胞被推测为II型和III型单个视锥细胞。在内段，特别是在高尔基体区域和粗面内质网中，未观察到染色。仅在连接纤毛以及油滴与面向染色严重的外段的质膜之间的小泡状或颗粒状结构中发现轻微染色。这表明视蛋白在内段远端部分被整合到质膜中。
