Aronin N, Morency K, Leeman S E, Braverman L E, Coslovsky R
Endocrinology. 1984 Jun;114(6):2138-42. doi: 10.1210/endo-114-6-2138.
The content of immunoreactive substance P (iSP) in the male rat anterior pituitary was measured after thyroidectomy and excess T4 administration. Baseline values for iSP content (mean +/- SE, 400 +/- 37 fmol/mg protein) increased progressively after thyroidectomy (4 days, 893 +/- 100; 6 days, 1321 +/- 242; 14 days, 1897 +/- 509). Administration of pharmacological doses of T4 (50 micrograms daily) for 2 and 14 days significantly decreased anterior pituitary iSP content (2 days, 196 +/- 30; 14 days 138 +/- 12). Thyroid status did not affect iSP content in the hypothalamus, caudate nucleus, amygdala, or brainstem. Partial chemical characterization of SP immunoreactivity in the anterior pituitary was obtained by gel permeation chromatography on Sephadex G-25, high pressure liquid chromatography, and the use of two antisera in RIAs, one directed against the amino-terminus and one directed against the carboxyl-terminus of the peptide. SP in the anterior pituitary was readily releasable in vitro by 44 mM potassium chloride in a calcium-dependent manner. The present study demonstrates that the concentration of iSP in the rat anterior pituitary is affected by the thyroid status of the animal and supports the probability that thyroid hormones participate in the regulation of the synthesis and/or release of iSP from the anterior pituitary.
在甲状腺切除和过量给予 T4 后，测定了雄性大鼠垂体前叶中免疫反应性 P 物质（iSP）的含量。甲状腺切除后（4 天，893±100；6 天，1321±242；14 天，1897±509），iSP 含量的基线值（平均值±标准误，400±37 fmol/mg 蛋白质）逐渐升高。给予药理剂量的 T4（每日 50 微克）2 天和 14 天，可显著降低垂体前叶 iSP 含量（2 天，196±30；14 天，138±12）。甲状腺状态不影响下丘脑、尾状核、杏仁核或脑干中的 iSP 含量。通过在 Sephadex G - 25 上进行凝胶渗透色谱、高压液相色谱以及在放射免疫分析中使用两种抗血清（一种针对肽的氨基末端，一种针对肽的羧基末端），对垂体前叶中 SP 免疫反应性进行了部分化学表征。垂体前叶中的 SP 在体外可被 44 mM 氯化钾以钙依赖的方式轻易释放。本研究表明，大鼠垂体前叶中 iSP 的浓度受动物甲状腺状态的影响，并支持甲状腺激素参与调节垂体前叶中 iSP 的合成和/或释放的可能性。
