Kruth H S, Fry D L
Exp Mol Pathol. 1984 Jun;40(3):288-94. doi: 10.1016/0014-4800(84)90046-7.
The fluorescent probe, filipin, and the lipid-soluble dye, oil red O, have been used to simultaneously detect and differentiate free and esterified cholesterol, respectively, in tissue sections prepared from spontaneous atherosclerotic lesions of swine. This was possible because filipin stains free cholesterol but does not stain cholesteryl ester and because oil red O stains cholesteryl ester but does not stain free cholesterol. Oil red O-stained lipid accumulated intra- and extracellularly but separate from filipin-stained lipid. Spherical filipin-stained particles and elongated filipin-stained crystals accumulated in the extracellular space. Interestingly, some cells appeared to accumulate these filipin-stained particles exclusively. The spherical filipin-stained particles have not been previously recognized because they are not stained by oil red O. This and the fact that extensive compartmentalization of filipin and oil red O-stained lipid occurs in atherosclerotic lesions are new observations to be considered in the pathogenesis of vascular cholesterol accumulation.
荧光探针制霉菌素和脂溶性染料油红O，已分别用于同时检测和区分猪自发性动脉粥样硬化病变组织切片中的游离胆固醇和酯化胆固醇。这之所以可行，是因为制霉菌素能使游离胆固醇染色，但不能使胆固醇酯染色，而油红O能使胆固醇酯染色，但不能使游离胆固醇染色。油红O染色的脂质在细胞内和细胞外积累，但与制霉菌素染色的脂质分开。球形的制霉菌素染色颗粒和细长的制霉菌素染色晶体在细胞外空间积累。有趣的是，一些细胞似乎只积累这些制霉菌素染色的颗粒。球形的制霉菌素染色颗粒以前未被识别，因为它们不能被油红O染色。制霉菌素和油红O染色的脂质在动脉粥样硬化病变中广泛分隔，这一事实是血管胆固醇积累发病机制中需要考虑的新观察结果。
