Panula P, Yang H Y, Costa E
J Comp Neurol. 1984 Apr 20;224(4):606-17. doi: 10.1002/cne.902240409.
Immunohistochemical localization of bombesin/gastrin-releasing peptide ( GRP )-like immunoreactivity (BN/ GRP -LI) and substance P-like immunoreactivity (SP-LI) in consecutive sections of rat hypothalamus was studied. Bombesin/ GRP -like immunoreactivity in the hypothalamus was partially characterized by gel filtration chromatography followed by radioimmunoassay. In the hypothalamus, SP-LI was more widely distributed than BN/ GRP -LI. Only the anterior and medial parvocellular parts of the nucleus paraventricularis and the nucleus suprachiasmaticus contained numerous cell bodies which exhibited BN/ GRP -LI. Neurons in these areas did not exhibit SP-LI. In contrast, cell bodies exhibiting SP-LI were numerous in the nucleus preopticus medialis and lateralis, nucleus anterior, nucleus ventromedialis and dorsomedialis, nucleus lateralis, nucleus arcuatus, and nucleus premamillaris ventralis and dorsalis. Only occasional cell bodies in these areas exhibited BN/ GRP -LI. It is concluded that the neuronal systems in the hypothalamus containing BN/ GRP -LI and SP-LI are separate, though the terminal fields in many areas overlap. Two peaks of BN/ GRP -LI were detected after gel filtration chromatography from extracts of the rat nucleus paraventricularis. The high molecular weight form coeluted with synthetic GRP (1-27), and the small molecular weight form eluted after synthetic bombesin. Thus, the endogenous BN/ GRP -LI is probably not authentic bombesin.
研究了大鼠下丘脑连续切片中蛙皮素/胃泌素释放肽（GRP）样免疫反应性（BN/GRP-LI）和P物质样免疫反应性（SP-LI）的免疫组织化学定位。下丘脑的蛙皮素/GRP样免疫反应性通过凝胶过滤色谱法继以放射免疫测定进行部分特性分析。在下丘脑中，SP-LI的分布比BN/GRP-LI更广泛。仅室旁核的前部和内侧小细胞部以及视交叉上核含有大量呈现BN/GRP-LI的细胞体。这些区域的神经元未呈现SP-LI。相反，呈现SP-LI的细胞体在视前内侧核和外侧核、前核、腹内侧核和背内侧核、外侧核、弓状核以及乳头体前腹侧核和背侧核中大量存在。这些区域仅偶尔有细胞体呈现BN/GRP-LI。得出的结论是，下丘脑含有BN/GRP-LI和SP-LI的神经元系统是分开的，尽管在许多区域的终末野重叠。从大鼠室旁核提取物经凝胶过滤色谱后检测到BN/GRP-LI的两个峰。高分子量形式与合成GRP（1-27）共洗脱，低分子量形式在合成蛙皮素之后洗脱。因此，内源性BN/GRP-LI可能不是真正的蛙皮素。
