Czitrom A A, Gascoigne N R, Edwards S, Waterfield D J
J Immunol. 1984 Jul;133(1):33-9.
Primary cytotoxic T lymphocyte (CTL) and helper T (Th) cell responses were generated from regional lymph nodes of heterozygous mice sensitized in the footpad with spleen cells of parental H-2 genotype but differing for multiple minor alloantigens of the B10 background. Cytotoxic and helper responses were read after a short in vitro culture period, which did not influence the restriction specificities of in vivo sensitized T cells. We show that under these conditions, Th cell responses appear unrestricted, whereas CTL responses are restricted to the immunizing parental H-2 genotype. Furthermore, we present evidence that these two functions are mediated by distinct T cell subsets and that the in vivo induction of Lyt-2- Th cells has the appearance of being unrestricted whereas that of Lyt-2+ CTL is restricted by H-2. These findings suggest that in a primary immune response, in vivo precursors of Th cells recognize processed antigen on F1 antigen-presenting cells (clones restricted to each parental H-2 genotype are cross-sensitized) under conditions in which those of CTL only respond directly to foreign minor alloantigens on the injected stimulator cells (clones restricted to parental H-2 genotypes are not cross-immunized). This dichotomy is taken to suggest the possibility that processing of cell membrane-bound antigens is controlled at their association step with products of the major histocompatibility complex.
用具有亲本H-2基因型但在B10背景的多个次要同种异体抗原方面存在差异的脾细胞对杂合小鼠的足垫进行致敏，从而从其局部淋巴结中产生了主要细胞毒性T淋巴细胞（CTL）和辅助性T（Th）细胞反应。在短时间的体外培养期后读取细胞毒性和辅助性反应，该培养期不会影响体内致敏T细胞的限制性特异性。我们发现，在这些条件下，Th细胞反应似乎不受限制，而CTL反应则限于免疫的亲本H-2基因型。此外，我们提供的证据表明，这两种功能由不同的T细胞亚群介导，并且Lyt-2-Th细胞的体内诱导似乎不受限制，而Lyt-2+CTL的诱导则受H-2限制。这些发现表明，在初次免疫反应中，Th细胞的体内前体在F1抗原呈递细胞上识别加工后的抗原（限于每种亲本H-2基因型的克隆被交叉致敏），而在这些条件下，CTL的前体仅直接对注射的刺激细胞上的外来次要同种异体抗原作出反应（限于亲本H-2基因型的克隆不会被交叉免疫）。这种二分法表明，细胞膜结合抗原的加工可能在其与主要组织相容性复合体产物的结合步骤受到控制。
