通过固相抗体形成细胞灶（AFCF）试验对大鼠同种特异性抗体产生细胞进行计数。

Enumeration of rat allospecific antibody-producing cells by a solid-phase antibody-forming cell focus (AFCF) assay.

作者信息

Majoor G D

出版信息

J Immunol Methods. 1984 Jun 8;71(1):37-42. doi: 10.1016/0022-1759(84)90203-5.

PMID:6202796

Abstract

Individual rat cells producing antibody to rat MHC class I alloantigens ( RT1A ) and other erythrocyte (E)-associated alloantigens can be enumerated by a solid-phase antibody-forming cell focus ( AFCF ) assay. Cells are incubated in solid agarose medium in petri dishes coated with affinity-purified anti-immunoglobulin antibodies and antibody produced by any cell is captured by these anti-immunoglobulin antibodies. Foci of antibody to E alloantigens produced by single cells become visible upon subsequent addition of the appropriate target E. Execution of the assay in solid agarose medium facilitates handling of the petri dishes and reduces target background binding compared to performance of the assay in liquid medium.

摘要

产生针对大鼠主要组织相容性复合体I类同种异体抗原（RT1A）及其他红细胞（E）相关同种异体抗原抗体的单个大鼠细胞，可通过固相抗体形成细胞集落（AFCF）测定法进行计数。将细胞接种于涂有亲和纯化抗免疫球蛋白抗体的培养皿中的固体琼脂糖培养基中，任何细胞产生的抗体都会被这些抗免疫球蛋白抗体捕获。在随后加入合适的靶E细胞后，单细胞产生的针对E同种异体抗原的抗体集落就会变得可见。与在液体培养基中进行该测定相比，在固体琼脂糖培养基中进行该测定便于培养皿的操作，并减少了靶背景结合。