Nakajima K, Hisazumi H, Kumaki O, Ueki O
Acta Radiol Oncol. 1983;22(6):487-92. doi: 10.3109/02841868309135975.
Using a clonogenic assay and the human bladder cancer cell line T24, enhanced cytotoxicity by the combined use of irradiation and 43 degrees C hyperthermia or two anticancer drugs, bleomycin (BLM) and cis-dichlorodiammineplatinum (CDDP), has been investigated in vitro, as well as the size of colonies derived from single survivor cells after treatment. Irradiation combined with 43 degrees C hyperthermia showed greatly enhanced cytotoxicity and diminution in mean colony diameter and decreased distribution of colony size. BLM had almost the same effect as 43 degrees C hyperthermia in terms of cytotoxicity and colony size. While CDDP showed a certain degree of enhanced cytotoxicity in combination with irradiation, it produced no appreciable decrease in mean colony diameter. Hyperthermia may be beneficial in the management of radiation resistant cells surviving a large dose of radiation, and would seem to be a promising means for achieving eradication of cancer cells in combination with radiation therapy.
利用克隆形成试验和人膀胱癌细胞系T24，在体外研究了联合使用辐射和43℃热疗或两种抗癌药物博来霉素（BLM）和顺二氯二氨铂（CDDP）增强的细胞毒性，以及处理后单个存活细胞衍生的集落大小。辐射联合43℃热疗显示出极大增强的细胞毒性、平均集落直径减小以及集落大小分布降低。就细胞毒性和集落大小而言，BLM与43℃热疗几乎具有相同的效果。虽然CDDP与辐射联合显示出一定程度的增强细胞毒性，但它并未使平均集落直径出现明显减小。热疗可能有益于处理在大剂量辐射后存活的抗辐射细胞，并且似乎是与放射治疗联合实现根除癌细胞的一种有前景的手段。
