Ogawa T, Takimori T, Takahashi Y
Brain Res. 1978 Jan 6;139(1):35-41. doi: 10.1016/0006-8993(78)90058-6.
Intracellular staining with recording micropipettes filled with a Procion yellow solution was attempted on 11 lateral geniculate neurons of chloralose-anesthetized cats for the purpose of correlating electrophysiologically identified cell types with morphologically identified cell types. Five neurons were successfully stained: one was located in lamina A and the others in lamina A1. They were all identified as P-cells on the basis of their responsiveness to electrical stimulation of optic disc, optic chiasm and visual cortex. The conduction velocities and synaptic delays for specific optic nerve fibers concerned with these P-cells were estimated from the relationship between latency and conduction distance. Intracellular staining has revealed that a neuron located in lamina A and three of four neurons located in lamina A1 had multipolar configuration, with their main dendrites oriented parallel to the plane of the laminae; while one neuron located in lamina A1 had a configuration similar to that of a class II-type neuron described by Guillery in his Golgi preparations. In these experiments no neurons located in lamina B, central interlaminar nucleus and medial interlaminar nucleus were stained. No interneurons were obtained, either.
为了将电生理鉴定的细胞类型与形态学鉴定的细胞类型相关联，我们尝试用充满普施安黄溶液的记录微电极对11只水合氯醛麻醉猫的外侧膝状体神经元进行细胞内染色。成功染色了5个神经元：1个位于A层，其他4个位于A1层。根据它们对视盘、视交叉和视觉皮层电刺激的反应，它们均被鉴定为P细胞。从潜伏期与传导距离的关系估算了与这些P细胞相关的特定视神经纤维的传导速度和突触延迟。细胞内染色显示，位于A层的1个神经元和位于A1层的4个神经元中的3个具有多极形态，其主要树突平行于板层平面；而位于A1层的1个神经元的形态类似于吉勒里在其高尔基染色标本中描述的II类神经元。在这些实验中，位于B层、中央层间核和内侧层间核的神经元均未被染色。也未获得中间神经元。
