Neumann D, Fridkin M, Fuchs S
Biochem Biophys Res Commun. 1984 Jun 15;121(2):673-9. doi: 10.1016/0006-291x(84)90234-1.
A synthetic peptide corresponding to the first twenty amino acids of the N-terminal region from the alpha-subunit of the Torpedo acetylcholine receptor cross reacts with antibodies to the receptor. A conjugate of this peptide to bovine serum albumin elicits in rabbits an immune response towards the synthetic peptide as well as towards the acetylcholine receptor. Blotting experiments demonstrate that the antipeptide antibodies react exclusively with the alpha-subunit of the acetylcholine receptor. Antibodies against synthetic peptides from various regions of the receptor sequence may provide useful reagents for structural and developmental analysis of the acetylcholine receptor as well as for the regulation of experimental autoimmune myasthenia gravis.
一种与电鳐乙酰胆碱受体α亚基N端区域前二十个氨基酸相对应的合成肽与该受体的抗体发生交叉反应。这种肽与牛血清白蛋白的缀合物在兔体内引发了针对合成肽以及乙酰胆碱受体的免疫反应。印迹实验表明，抗肽抗体仅与乙酰胆碱受体的α亚基发生反应。针对受体序列不同区域的合成肽产生的抗体可能为乙酰胆碱受体的结构和发育分析以及实验性自身免疫性重症肌无力的调控提供有用的试剂。
