兔抗同源肝NADPH-细胞色素c（P-450）还原酶抗体对豚鼠多形核白细胞产生超氧阴离子的NADPH氧化酶的抑制作用。

Inhibition of the O2- -generating NADPH oxidase of guinea-pig polymorphonuclear leukocytes by rabbit antibody to homologous liver NADPH-cytochrome c (P-450) reductase.

作者信息

Takayama H, Iwaki S, Tamoto K, Koyama J

出版信息

Biochim Biophys Acta. 1984 Jun 15;799(2):151-7. doi: 10.1016/0304-4165(84)90289-7.

PMID:6203553

Abstract

Rabbit antibody highly specific for guinea-pig liver NADPH-cytochrome c (P-450) reductase was found to inhibit dose-dependently the O2- -generating activity of the membrane fraction isolated from phorbol-myristate acetate-stimulated, homologous polymorphonuclear leukocytes. In addition, the antibody also could inhibit the NADPH-cytochrome c (Nitroblue tetrazolium) reductase separated from the membrane fractions and phagosomes of leukocytes by polyacrylamide gel electrophoresis or gel filtration on a Sephacryl S-300 column in the presence of 0.2% Triton X-100. These results demonstrate that the NADPH-cytochrome c reductase in the membrane fractions of leukocytes is antigenically cross-reactive with homologous liver NADPH-cytochrome c reductase, and also suggest that the enzyme of leukocytes participates in the respiratory burst.

摘要

发现对豚鼠肝脏NADPH-细胞色素c（P-450）还原酶具有高度特异性的兔抗体可剂量依赖性地抑制从佛波醇-肉豆蔻酸酯乙酸酯刺激的同源多形核白细胞中分离出的膜部分的O2-生成活性。此外，在0.2% Triton X-100存在的情况下，该抗体还可通过聚丙烯酰胺凝胶电泳或在Sephacryl S-300柱上进行凝胶过滤，抑制从白细胞的膜部分和吞噬体中分离出的NADPH-细胞色素c（硝基蓝四唑）还原酶。这些结果表明，白细胞膜部分中的NADPH-细胞色素c还原酶与同源肝脏NADPH-细胞色素c还原酶具有抗原交叉反应性，也表明白细胞的该酶参与呼吸爆发。