Bernadac A, Gorvel J P, Feracci H, Maroux S
Biol Cell. 1984;50(1):31-6. doi: 10.1111/j.1768-322x.1984.tb00252.x.
Human blood group A antigenicity of glycoproteins is retained on epon-embedded jejunum sections after glutaraldehyde fixation and osmium treatment. The intracellular location of molecules bearing these determinants was visualized in the four types of epithelial cells of A+ rabbit jejunum sections with immuno-colloidal gold labeling. The brush border membrane and in particular the glycocalyx of absorbing cells as well as the secretory granules of goblet and Paneth cells were heavily labeled. In enteroendocrine cells, the membrane of secretory granules and not their content was lightly labeled. The differential labeling of secretory or membrane bound glycoproteins is accompanied by different labels of the Golgi complex as expected if labeling of the Golgi saccules was due to the presence of glycoproteins in transit. In all cases the label is primarily concentrated in only half the cisternae on the trans side of the Golgi stacks. In absorbing cells, structures have been revealed in the terminal web that could be related to the brush border membrane and consequently implicated in its biogenesis. The fibrillar material of the glycocalyx appears as highly labeled tangled structures which apparently proceed from densely stained "carrier" vesicles arising from the Golgi apparatus. Vesicles fusing at the lower part of microvilli could result of integration of this material into the lightly labeled vesicles strictly found in the terminal web. These last vesicles could also contain newly synthesized brush border hydrolases.
戊二醛固定和锇处理后，糖蛋白的人血型A抗原性保留在环氧树脂包埋的空肠切片上。用免疫胶体金标记法在A+兔空肠切片的四种上皮细胞中观察到携带这些决定簇的分子的细胞内定位。刷状缘膜，特别是吸收细胞的糖萼以及杯状细胞和潘氏细胞的分泌颗粒被大量标记。在内分泌细胞中，分泌颗粒的膜而非其内容物被轻度标记。分泌性或膜结合糖蛋白的差异标记伴随着高尔基体的不同标记，如果高尔基体囊泡的标记是由于转运中的糖蛋白的存在，这是预期的。在所有情况下，标记主要集中在高尔基体堆叠反面膜囊的仅一半中。在吸收细胞中，终末网中发现了一些结构，这些结构可能与刷状缘膜有关，因此与刷状缘膜的生物发生有关。糖萼的纤维状物质表现为高度标记的缠结结构，这些结构显然来自高尔基体产生的深色“载体”囊泡。在微绒毛下部融合的囊泡可能是这种物质整合到终末网中严格发现的轻度标记的囊泡中的结果。这些最后的囊泡也可能含有新合成的刷状缘水解酶。
