McQueen J K, Martin M J, Harmar A J
Brain Res. 1984 May 21;300(1):19-26. doi: 10.1016/0006-8993(84)91336-2.
The effects of the steroid anaesthetic Althesin (alphaxalone plus alphadolone acetate) on regional cerebral metabolism was studied in female rats. [14C]2-Deoxyglucose (2-DG) uptake was measured in 19 discrete anatomical areas by quantitative autoradiography. Under Althesin anaesthesia metabolic activity, relative to the corpus callosum ( rma ), was significantly (24-46%) increased in the locus coeruleus, medial (but not lateral) habenula (Hb) and interpeduncular nucleus (IPN). The Hb-IPN tract, not discernible in autoradiograms from conscious rats, became readily apparent in films from anaesthetized rats. However, the increased metabolic activity of this pathway was not associated with a significant change in the tissue concentrations of substance P in either the Hb or IPN. In sensorimotor and visual cortex, caudate nucleus, thalamic nuclei and the medial geniculate body rma was significantly (26-38%) depressed. Metabolic activity in the other 8 areas measured was unaffected by Althesin. Destruction of the stria medullaris input to the habenulae prevented the Althesin-induced increase in 2-DG uptake by the MHb and LHb.
在雌性大鼠中研究了类固醇麻醉剂Althesin（α-羟孕酮与醋酸α-孕烷醇酮）对局部脑代谢的影响。通过定量放射自显影术测量了19个离散解剖区域的[¹⁴C]2-脱氧葡萄糖（2-DG）摄取。在Althesin麻醉下，相对于胼胝体（rma），蓝斑、内侧（而非外侧）缰核（Hb）和脚间核（IPN）的代谢活性显著增加（24%-46%）。在清醒大鼠的放射自显影片中无法分辨的Hb-IPN束，在麻醉大鼠的片子中变得清晰可见。然而，该通路代谢活性的增加与Hb或IPN中P物质的组织浓度的显著变化无关。在感觉运动和视觉皮层、尾状核、丘脑核团和内侧膝状体中，rma显著降低（26%-38%）。所测量的其他8个区域的代谢活性不受Althesin影响。破坏输入到缰核的髓纹可防止Althesin诱导的内侧缰核和外侧缰核2-DG摄取增加。
