Hoefer M T, Heiman M L, Ben-Jonathan N
Mol Cell Endocrinol. 1984 May;35(2-3):229-35. doi: 10.1016/0303-7207(84)90021-2.
The characteristics of prolactin (PRL) secretion by cultured anterior pituitary cells in the presence and absence of catecholamines were studied. PRL secretion was markedly influenced by culture conditions such as cell density, culture duration and length of short-term incubation. Dopamine (DA) inhibited PRL release in a dose-dependent manner within a physiological range, and this inhibition was reversed by the stereospecific DA receptor antagonist (+)-butaclamol. In contrast, the inhibition of PRL secretion by norepinephrine (NE) required much higher doses and lacked specificity. Cells obtained from male donors had the lowest basal PRL secretion and were the least responsive to DA inhibition, whereas those obtained from females in late pregnancy had the highest basal PRL secretion and were the most sensitive to DA.
研究了在有无儿茶酚胺存在的情况下，培养的垂体前叶细胞催乳素（PRL）分泌的特征。PRL分泌受到培养条件的显著影响，如细胞密度、培养持续时间和短期孵育时长。多巴胺（DA）在生理范围内以剂量依赖方式抑制PRL释放，且这种抑制作用可被立体特异性DA受体拮抗剂（+）-布他拉莫逆转。相比之下，去甲肾上腺素（NE）对PRL分泌的抑制需要更高剂量且缺乏特异性。来自雄性供体的细胞基础PRL分泌最低，对DA抑制的反应也最小，而来自妊娠晚期雌性的细胞基础PRL分泌最高，对DA最敏感。
