Wang Y, Joncourt F, Kristensen F, de Weck A L
Int J Immunopharmacol. 1984;6(2):105-10. doi: 10.1016/0192-0561(84)90004-3.
Enriched human peripheral T-lymphocytes were stimulated with PHA and examined for variations in insulin and glucocorticoid (dexamethasone) receptor numbers during the early phases of the cell cycle. Cells in G0, G1a and G1b phases, where the G1a - G1b transition is an Interleukin 2 dependent event, were quantitated by flow cytometry. Few but significant numbers of glucocorticoid receptors (2700/cell) and no insulin receptors (-1/cell) were found in the resting (G0) phase. As cells entered the G1a phase the specific binding of dexamethasone increased and of insulin took place. Although the specific binding further increased as T-cells entered the G1b phase (as measured at 44 h of incubation and using hydroxyurea-treated cells), the major changes in the specific binding of dexamethasone took place during the period 16 - 20 h after stimulation. Based on these findings, it is concluded that both receptor types (cell membrane and cytoplasmic receptors) are being formed and increased at G1 phase prior to cell proliferation, indicating the importance of G1 phase in immunoregulation.
用植物血凝素（PHA）刺激富集的人外周血T淋巴细胞，并检测细胞周期早期胰岛素和糖皮质激素（地塞米松）受体数量的变化。通过流式细胞术对处于G0、G1a和G1b期的细胞进行定量，其中G1a - G1b期的转换是白细胞介素2依赖性事件。在静止（G0）期发现少量但数量可观的糖皮质激素受体（2700个/细胞），未发现胰岛素受体（-1个/细胞）。随着细胞进入G1a期，地塞米松的特异性结合增加，胰岛素的特异性结合开始出现。尽管随着T细胞进入G1b期（在培养44小时时测量，并使用羟基脲处理的细胞），特异性结合进一步增加，但地塞米松特异性结合的主要变化发生在刺激后16 - 20小时。基于这些发现，可以得出结论，两种受体类型（细胞膜受体和细胞质受体）在细胞增殖前的G1期形成并增加，表明G1期在免疫调节中的重要性。
